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Из растения Peganum harmala L. выделены вазицинон и пеганин. Получены 2,4-динитрофенилгидразон вазицинона, кеталь вазицинона. Хиназоли...
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Из растения Peganum harmala L. выделены вазицинон и пеганин. Получены 2,4-динитрофенилгидразон вазицинона, кеталь вазицинона. Хиназолиновые алкалоиды - действующее начало популярных в народной медицине растений сем. гармаловых (Peganaceae Tiegh.) [1-3]. Мы исследовали алкалоиды гармалы обыкновенной (Peganum harmala L.), собранной в Карагандинской области (Казахстан) в фазу цветения - плодоношения.
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Peganum lzarmala L. seeds were germinated in hormone free sterile MS medium. Root, shoot tip, leaf, petiole, hypocotyl, cotyledon and nod explants excised from in. vitro regenerated plants were incubated in MS medium supplemented ...
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Peganum lzarmala L. seeds were germinated in hormone free sterile MS medium. Root, shoot tip, leaf, petiole, hypocotyl, cotyledon and nod explants excised from in. vitro regenerated plants were incubated in MS medium supplemented with 1 mg/l and 2 mg/l 2,4-D for callus production. Hypo- cotyl and cotyledon explants produced callus and untreated callus was accepted as control for the comparison of [3-carboline alkaloids production extracted from different samples. Callus were ex- posed to cold and dark conditions and incubated in MS medium supplemented with 2 mg/l ABA, 1 mg/l ABA, 1 mg/l NAA and 1 mg/l BAP in differ- ent batches to increase [3-carboline alkaloid produc- tion. Seeds were germinated in soil ambient condi- tion. [3-carboline alkaloids; harmalol, harmine and harmaline were extracted from seeds, plants grown in soil, callus grown in MS medium supplemented with 2,4-D, callus incubated MS medium supple- mented with NAA, BAP, ABA and callus exposed cold and dark condition. The extracts were quantita- tively analysed with HPLC. The highest alkaloid concentrations were determined in. vivo seeds and plants grown in soil. However, alkaloid production in callus tissues is also promising. Harmalol and harmine concentration decreased with cold treat- ment but increased with ABA treatment compared to that of the control. Harmaline was increased with cold treatment, but dramatically decreased in all treatment that callus is exposed.
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Peganum harmala L. is a medicinal herb extensively used in traditional Chinese medicine (TCM). So far, relevant reports on the toxicity of Peganum harmala L. seeds (PHS) are hardly available. Especially, we still know little about...
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Peganum harmala L. is a medicinal herb extensively used in traditional Chinese medicine (TCM). So far, relevant reports on the toxicity of Peganum harmala L. seeds (PHS) are hardly available. Especially, we still know little about the in vivo mechanism for PHS toxicity. This study aims to evaluate the toxicity effects of PHS in Caenorhabditis elegans (C. elegans), investigate the possible mechanism of the toxicity effects of PHS, and provide reference for the pharmacological research of PHS. In the present study, the C. elegans was exposed to 0.25, 0.50, 1.00?mg/mL of PHS in nematode growth medium (NGM) at 22?°C in the presence of food. Lethality, lifespan, growth, reproduction, and locomotion behavior assays were performed to evaluate the toxicity effects of PHS in C. elegans. We then determined the mechanism of the toxicity effect of PHS by quantitative real-time polymerase chain reaction (qRT-PCR), acetylcholinesterase (AChE) activity assay, and oxidative stress resistance assays. The main components of PHS were detected by high performance liquid chromatography (HPLC). Compared with the control group, the lethality of C. elegans was significantly increased when they were exposed to the ethanol extract of PHS at 0.25, 0.50 and 1.00?mg/mL (P?收起
摘要 :
Peganum harmala L. is a medicinal herb extensively used in traditional Chinese medicine (TCM). So far, relevant reports on the toxicity of Peganum harmala L. seeds (PHS) are hardly available. Especially, we still know little about...
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Peganum harmala L. is a medicinal herb extensively used in traditional Chinese medicine (TCM). So far, relevant reports on the toxicity of Peganum harmala L. seeds (PHS) are hardly available. Especially, we still know little about the in vivo mechanism for PHS toxicity. This study aims to evaluate the toxicity effects of PHS in Caenorhabditis elegans (C. elegans), investigate the possible mechanism of the toxicity effects of PHS, and provide reference for the pharmacological research of PHS. In the present study, the C. elegans was exposed to 0.25, 0.50, 1.00?mg/mL of PHS in nematode growth medium (NGM) at 22?°C in the presence of food. Lethality, lifespan, growth, reproduction, and locomotion behavior assays were performed to evaluate the toxicity effects of PHS in C. elegans. We then determined the mechanism of the toxicity effect of PHS by quantitative real-time polymerase chain reaction (qRT-PCR), acetylcholinesterase (AChE) activity assay, and oxidative stress resistance assays. The main components of PHS were detected by high performance liquid chromatography (HPLC). Compared with the control group, the lethality of C. elegans was significantly increased when they were exposed to the ethanol extract of PHS at 0.25, 0.50 and 1.00?mg/mL (P?<?0.01), and the mean lifespan was significantly decreased (P?<?0.01). We also observed that PHS exposure could induce the toxicity on body length, brood size, and locomotion behavior. Our study shows that the ethanol extract of PHS exerts obvious toxic effects on C. elegans, which would provide new ideas and methods for the biological evaluation of the toxicity of Chinese medicinal materials.
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Peganum harmala L was used traditionally in different populations for many medical complains. It contained a wide range of chemical constituents.. The previous studies showed that the seeds of the plant and its constituents exerte...
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Peganum harmala L was used traditionally in different populations for many medical complains. It contained a wide range of chemical constituents.. The previous studies showed that the seeds of the plant and its constituents exerted antimicrobial, anticancer, antioxidant, antidiabetic and analgesic effects and many other pharmacological activities. Harmaline, harmine, harmalol, harman, quinazoline derivatives, vasicine, vasicinone, anthroquinons and fixed oils are reported from seeds and roots of this plant. This plant is used as a medicine in Turkey, Syria, Iran, Pakistan, India, Egypt and Spain. This article presents comprehensive analyzed information on the botanical, chemical and pharmacological aspects of P. harmala.
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Peganum harmala belong to the Jigo phalluses family has many compounds as alkaloids, Saponines steroids and lignin which are used as a medicinal components which serve as a regulator to endocrine activity, in this study, twenty ad...
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Peganum harmala belong to the Jigo phalluses family has many compounds as alkaloids, Saponines steroids and lignin which are used as a medicinal components which serve as a regulator to endocrine activity, in this study, twenty adult female local rabbits with weighing 1500±100gms, and aged 240 ±10 days were divided into 2 groups: the control group which fed on diet and water ad libitum and the treatment group which administrated orally (by stomach tube) 10cc with 13.5% of peganum harmala alcoholic extract daily for 14 consecutive days, and at the 15th day, blood samples were collected. Serum level of Triiodothyronin (T3), Thyroxin (T4) and Thyroid-stimulating hormone (TSH), uric acid and creatinine were measured by using radioimmunoassay method. Results were revealed that the 90mg/kg dosage of peganum harmala alcoholic extract increased significantly (p<0.05) the levels of the urea and uric acid when compared with control, while the creatinine has not recorded significant variances when compared with the control group, on the other hand, the effect of Peganum harmala seed alcoholic extract on the TSH, T3, T4 levels revealed that these hormones decreased significantly (p<0.05) when compared with the control group. In conclusion the results of this study indicate that the 90mg/kg of alcoholic extract of Peganum harmala seeds has increased blood urea and uric acid, decreased blood TSH as well as hormones of thyroid gland.
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The use of traditional medicines as a diuretic agent has been increasing in recent years. The diuretic activity of a number of plant extracts used as diuretic agents in ethnomedicine has been confirmed in experimental animals. How...
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The use of traditional medicines as a diuretic agent has been increasing in recent years. The diuretic activity of a number of plant extracts used as diuretic agents in ethnomedicine has been confirmed in experimental animals. However, despite the widespread use of Peganum harmala in traditional medicine, there is a paucity of data supporting its use as a diuretic agent. Therefore, the present study aimed to envisage the true effect and magnitude of diuresis of methanolic extract of P. harmala (MEPH) in comparison with a well-known diuretic drug furosemide using Wistar albino rats. MEPH was administered orally in three different doses (150, 300 and 450mg/kg) to experimentally dehydrated rats. Furosemide (10mg/kg orally) was used as a reference drug. The diuretic effect of the MEPH was evaluated by measuring urine volume, urine pH, urinary electrolyte levels, natriuretic and saliuretic effects. The urine volume (in mL) measured at 5h and 24h and electrolyte excretion (Na^+, K^+, and Cl^-) at 24h duration were measured. The urine output and urinary electrolyte excretion were found to be significantly higher in rats treated with MEPH as compared to normal rats in a dose dependent manner (P<0.05). The results of our study were comparable to furosemide drug. Based on observed results, we can recommend that P. harmala may be an effective diuretic, however, toxicity studies should be conducted before administration.
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Graphical abstract Display Omitted Abstract Seventeen quinazoline alkaloids and derivatives, containing two pairs of new epimers, named as ( S )- and ( R )-1-(2-aminobenzyl)-3-hydroxypyrrolidin-2-one β - d -glucopyranosyl-(1?→?6...
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Graphical abstract Display Omitted Abstract Seventeen quinazoline alkaloids and derivatives, containing two pairs of new epimers, named as ( S )- and ( R )-1-(2-aminobenzyl)-3-hydroxypyrrolidin-2-one β - d -glucopyranosyl-(1?→?6)- β - d -glucopyranoside ( 1 , 2 ), ( S )- and ( R )-vasicinone β - d -glucopyranosyl-(1?→?6)- β - d -glucopyranoside ( 3 , 4 ), and a new enantiomer ( 12b ), together with six known ones ( 5 – 8 , 10 , and 12a ), and three pairs of known enantiomers ( 9 , 11 , and 13 ), were isolated from the ethanol extracts of the seeds of Peganum harmala L.. Their structures including the absolute configuration were elucidated by using 1D and 2D NMR, and ECD calculation approaches. The cytotoxic activities of all isolated compounds were evaluated. 11 showed moderate cytotoxicity against PC-3 cells with an IC 50 value of 15.41?μM.
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This study was designed to evaluate the role of Peganum harmala extract on experimental skin wound healing. The investigation was carried out on 30 male Sprague-Dawley rats in the same conditions. Two uniform 7-mm diameter skin de...
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This study was designed to evaluate the role of Peganum harmala extract on experimental skin wound healing. The investigation was carried out on 30 male Sprague-Dawley rats in the same conditions. Two uniform 7-mm diameter skin defect were created on the back of each animal by skin punch (total of 60 wounds). The extract was then applied once daily on half of the wounds. The animals were sacrificed on day 10 for histopathologic and biomechanical examinations. P. harmala extract significantly increased the number of fibroblasts and capillary buds and also decreased the epithelial gap which showed the better healing in treatment group. On the other hand, the improvement of biomechanical indices in treatment group revealed a significant increase in tensile strength of the wounds. It can be concluded that P. harmala is an effective herbal remedy in wound healing
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The beta-carboline alkaloids of the harmala (HAlks) group are compounds widely spread in many natural sources, but found at relatively high levels in some specific plants like Peganum harmala (Syrian rue) or Banisteriopsis caapi. ...
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The beta-carboline alkaloids of the harmala (HAlks) group are compounds widely spread in many natural sources, but found at relatively high levels in some specific plants like Peganum harmala (Syrian rue) or Banisteriopsis caapi. HAlks are a reversible Mono Amino Oxidase type A Inhibitor (MAOI) and, as a consequence, these plants or their extracts can be used to produce psychotropic effects when are combined with psychotropic drugs based on amino groups. Since the occurrence and the levels of the HAlks in natural sources are subject to significant variability, more widespread use is not clinical but recreational or ritual, for example B. caapi is a known part of the Ayahuasca ritual mixture. The lack of simple methods to control the variable levels of these compounds in natural sources restricts the possibilities to dose in strict quantities and, as a consequence, limits its use with pharmacological or clinical purposes. In this work, we present a fast, simple, and robust method of quantifying simultaneously the six HAlks more frequently found in plants, i.e., harmine, harmaline, harmol, harmalol, harmane, and norharmane, by capillary electrophoresis instruments equipped with the more common detector UV. The method is applied to analyze these HAlks in P. Harmala seeds infusion which is a frequent intake form for these HAlks. The method is validated in three different instruments in order to evaluate the transferability and to compare the performances between them. In this case, harmaline, harmine, and harmol were found in the infusion samples. Copyright (c) 2016 John Wiley & Sons, Ltd.
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